( B) Growth kinetics of Colo205 NLR tumor cells co-cultured with mock, control, or LY元31 samples was assessed over a 114-hr time course using IncuCyte. Data represent the mean value from duplicate measurements for each sample. Killing curves are shown for the mock (black), control (EF1 α_NY-ESO-1 red), and LY元31 (EF1 α_c-JunWT_NY-ESO-1 green) samples from one representative donor. ( A) Cytotoxic capacity was assessed over a 168-hr time course of co-culture with H1703 or A375 NLR tumor cells using an IncuCyte™ S3 Live-Cell Analysis System. Functional activity of LY元31 and control T cells was evaluated against NY-ESO-1antigen-positive tumor cell lines (H1703 and A375) or an NY-ESO-1antigen-negative tumor cell line (Colo205, negative control) transduced to stably express NucLightRed (NLR) at E:T ratios of 1:1, 1:5, 1:10, or 1:20.
